Flow Cytometry:Article Title: Splenectomy reduces shear stress and inflammation in liver endothelial cells during regeneration after partial hepatectomy in mice.
Article Snippet: .. Flow cytometry A 105 aliquote of LSECs isolated using magnetic sorting for the CD146 marker were incubated in 100 μl Rinsing Solution (Miltenyi Biotec, USA) with 5 μl primary antibodies to CD31 (PECAM-1) (PE-labeled, 130-111-540, clone REA784 | 390, Miltenyi Biotec, USA), integrin alpha-5 (CD49e) (PE-labeled, 130-122-072, AR TIC LE IN PR ES S clone REA1183 | 5H10-27, Miltenyi Biotec, USA), VCAM-1 (CD106) (PE-labeled, 130-116-323, clone REA971 | 429, Miltenyi Biotec, USA), VE-cadherin (CD144) (PE-labeled, 130-128-207, clone REA225 | BV13, Miltenyi Biotec, USA), Ki67 (FITC-labeled, 130- 117-691, clone REA183 | B56 Miltenyi Biotec, USA), CD146 (130- 102-230, clone ME-9F1, Miltenyi Biotec, USA), F4/80 (130-102- 422, REA126 | BM8, Miltenyi Biotec, USA ) at room temperature for 1 hour. .. The dynamics of CD3+ lymphocytes and NK1.1 cells in regenerating livers were analyzed similarly using 105 cells of the liver stromal fraction and primary antibodies to CD45 (PE-labeled, 130-102-596, clone 30F11, Miltenyi Biotec, USA), CD3 (APClabeled, MCA500APC, clone KT3, Bio-Rad USA), NK1.1 (VioBluelabeled, Biolegend, USA); the incubations proceeded for 1 hour.
Article Title: Splenectomy reduces shear stress and inflammation in liver endothelial cells during regeneration after partial hepatectomy in mice
Article Snippet: .. A 10 5 aliquote of LSECs isolated using magnetic sorting for the CD146 marker were incubated in 100 μl Rinsing Solution (Miltenyi Biotec, USA) with 5 μl primary antibodies to CD31 (PECAM-1) (PE-labeled, 130–111-540, clone REA784 | 390, Miltenyi Biotec, USA), integrin alpha-5 (CD49e) (PE-labeled, 130–122-072, clone REA1183 | 5H10-27, Miltenyi Biotec, USA), VCAM-1 (CD106) (PE-labeled, 130–116-323, clone REA971 | 429, Miltenyi Biotec, USA), VE-cadherin (CD144) (PE-labeled, 130–128-207, clone REA225 | BV13, Miltenyi Biotec, USA), Ki67 (FITC-labeled, 130–117-691, clone REA183 | B56 Miltenyi Biotec, USA), CD146 (130–102-230, clone ME-9F1, Miltenyi Biotec, USA), F4/80 (130–102-422, REA126 | BM8, Miltenyi Biotec, USA ) at room temperature for 1 h. The dynamics of CD3 + lymphocytes and NK1.1 cells in regenerating livers were analyzed similarly using 10 5 cells of the liver stromal fraction and primary antibodies to CD45 (PE-labeled, 130–102-596, clone 30F11, Miltenyi Biotec, USA), CD3 (APC-labeled, MCA500APC, clone KT3, Bio-Rad USA), NK1.1 (VioBlue-labeled, Biolegend, USA); the incubations proceeded for 1 h. Following the incubations, the cells were washed in PBS, resuspended in 0.5 ml PBS and analyzed in a MACSQuant 10 flow cytometer (Milteniy Biotech, Germany); the data were analyzed in FlowJo (LLC). ..
Isolation:Article Title: Splenectomy reduces shear stress and inflammation in liver endothelial cells during regeneration after partial hepatectomy in mice.
Article Snippet: .. Flow cytometry A 105 aliquote of LSECs isolated using magnetic sorting for the CD146 marker were incubated in 100 μl Rinsing Solution (Miltenyi Biotec, USA) with 5 μl primary antibodies to CD31 (PECAM-1) (PE-labeled, 130-111-540, clone REA784 | 390, Miltenyi Biotec, USA), integrin alpha-5 (CD49e) (PE-labeled, 130-122-072, AR TIC LE IN PR ES S clone REA1183 | 5H10-27, Miltenyi Biotec, USA), VCAM-1 (CD106) (PE-labeled, 130-116-323, clone REA971 | 429, Miltenyi Biotec, USA), VE-cadherin (CD144) (PE-labeled, 130-128-207, clone REA225 | BV13, Miltenyi Biotec, USA), Ki67 (FITC-labeled, 130- 117-691, clone REA183 | B56 Miltenyi Biotec, USA), CD146 (130- 102-230, clone ME-9F1, Miltenyi Biotec, USA), F4/80 (130-102- 422, REA126 | BM8, Miltenyi Biotec, USA ) at room temperature for 1 hour. .. The dynamics of CD3+ lymphocytes and NK1.1 cells in regenerating livers were analyzed similarly using 105 cells of the liver stromal fraction and primary antibodies to CD45 (PE-labeled, 130-102-596, clone 30F11, Miltenyi Biotec, USA), CD3 (APClabeled, MCA500APC, clone KT3, Bio-Rad USA), NK1.1 (VioBluelabeled, Biolegend, USA); the incubations proceeded for 1 hour.
Article Title: Splenectomy reduces shear stress and inflammation in liver endothelial cells during regeneration after partial hepatectomy in mice
Article Snippet: .. A 10 5 aliquote of LSECs isolated using magnetic sorting for the CD146 marker were incubated in 100 μl Rinsing Solution (Miltenyi Biotec, USA) with 5 μl primary antibodies to CD31 (PECAM-1) (PE-labeled, 130–111-540, clone REA784 | 390, Miltenyi Biotec, USA), integrin alpha-5 (CD49e) (PE-labeled, 130–122-072, clone REA1183 | 5H10-27, Miltenyi Biotec, USA), VCAM-1 (CD106) (PE-labeled, 130–116-323, clone REA971 | 429, Miltenyi Biotec, USA), VE-cadherin (CD144) (PE-labeled, 130–128-207, clone REA225 | BV13, Miltenyi Biotec, USA), Ki67 (FITC-labeled, 130–117-691, clone REA183 | B56 Miltenyi Biotec, USA), CD146 (130–102-230, clone ME-9F1, Miltenyi Biotec, USA), F4/80 (130–102-422, REA126 | BM8, Miltenyi Biotec, USA ) at room temperature for 1 h. The dynamics of CD3 + lymphocytes and NK1.1 cells in regenerating livers were analyzed similarly using 10 5 cells of the liver stromal fraction and primary antibodies to CD45 (PE-labeled, 130–102-596, clone 30F11, Miltenyi Biotec, USA), CD3 (APC-labeled, MCA500APC, clone KT3, Bio-Rad USA), NK1.1 (VioBlue-labeled, Biolegend, USA); the incubations proceeded for 1 h. Following the incubations, the cells were washed in PBS, resuspended in 0.5 ml PBS and analyzed in a MACSQuant 10 flow cytometer (Milteniy Biotech, Germany); the data were analyzed in FlowJo (LLC). ..
Marker:Article Title: Splenectomy reduces shear stress and inflammation in liver endothelial cells during regeneration after partial hepatectomy in mice.
Article Snippet: .. Flow cytometry A 105 aliquote of LSECs isolated using magnetic sorting for the CD146 marker were incubated in 100 μl Rinsing Solution (Miltenyi Biotec, USA) with 5 μl primary antibodies to CD31 (PECAM-1) (PE-labeled, 130-111-540, clone REA784 | 390, Miltenyi Biotec, USA), integrin alpha-5 (CD49e) (PE-labeled, 130-122-072, AR TIC LE IN PR ES S clone REA1183 | 5H10-27, Miltenyi Biotec, USA), VCAM-1 (CD106) (PE-labeled, 130-116-323, clone REA971 | 429, Miltenyi Biotec, USA), VE-cadherin (CD144) (PE-labeled, 130-128-207, clone REA225 | BV13, Miltenyi Biotec, USA), Ki67 (FITC-labeled, 130- 117-691, clone REA183 | B56 Miltenyi Biotec, USA), CD146 (130- 102-230, clone ME-9F1, Miltenyi Biotec, USA), F4/80 (130-102- 422, REA126 | BM8, Miltenyi Biotec, USA ) at room temperature for 1 hour. .. The dynamics of CD3+ lymphocytes and NK1.1 cells in regenerating livers were analyzed similarly using 105 cells of the liver stromal fraction and primary antibodies to CD45 (PE-labeled, 130-102-596, clone 30F11, Miltenyi Biotec, USA), CD3 (APClabeled, MCA500APC, clone KT3, Bio-Rad USA), NK1.1 (VioBluelabeled, Biolegend, USA); the incubations proceeded for 1 hour.
Article Title: Splenectomy reduces shear stress and inflammation in liver endothelial cells during regeneration after partial hepatectomy in mice
Article Snippet: .. A 10 5 aliquote of LSECs isolated using magnetic sorting for the CD146 marker were incubated in 100 μl Rinsing Solution (Miltenyi Biotec, USA) with 5 μl primary antibodies to CD31 (PECAM-1) (PE-labeled, 130–111-540, clone REA784 | 390, Miltenyi Biotec, USA), integrin alpha-5 (CD49e) (PE-labeled, 130–122-072, clone REA1183 | 5H10-27, Miltenyi Biotec, USA), VCAM-1 (CD106) (PE-labeled, 130–116-323, clone REA971 | 429, Miltenyi Biotec, USA), VE-cadherin (CD144) (PE-labeled, 130–128-207, clone REA225 | BV13, Miltenyi Biotec, USA), Ki67 (FITC-labeled, 130–117-691, clone REA183 | B56 Miltenyi Biotec, USA), CD146 (130–102-230, clone ME-9F1, Miltenyi Biotec, USA), F4/80 (130–102-422, REA126 | BM8, Miltenyi Biotec, USA ) at room temperature for 1 h. The dynamics of CD3 + lymphocytes and NK1.1 cells in regenerating livers were analyzed similarly using 10 5 cells of the liver stromal fraction and primary antibodies to CD45 (PE-labeled, 130–102-596, clone 30F11, Miltenyi Biotec, USA), CD3 (APC-labeled, MCA500APC, clone KT3, Bio-Rad USA), NK1.1 (VioBlue-labeled, Biolegend, USA); the incubations proceeded for 1 h. Following the incubations, the cells were washed in PBS, resuspended in 0.5 ml PBS and analyzed in a MACSQuant 10 flow cytometer (Milteniy Biotech, Germany); the data were analyzed in FlowJo (LLC). ..
Incubation:Article Title: Splenectomy reduces shear stress and inflammation in liver endothelial cells during regeneration after partial hepatectomy in mice.
Article Snippet: .. Flow cytometry A 105 aliquote of LSECs isolated using magnetic sorting for the CD146 marker were incubated in 100 μl Rinsing Solution (Miltenyi Biotec, USA) with 5 μl primary antibodies to CD31 (PECAM-1) (PE-labeled, 130-111-540, clone REA784 | 390, Miltenyi Biotec, USA), integrin alpha-5 (CD49e) (PE-labeled, 130-122-072, AR TIC LE IN PR ES S clone REA1183 | 5H10-27, Miltenyi Biotec, USA), VCAM-1 (CD106) (PE-labeled, 130-116-323, clone REA971 | 429, Miltenyi Biotec, USA), VE-cadherin (CD144) (PE-labeled, 130-128-207, clone REA225 | BV13, Miltenyi Biotec, USA), Ki67 (FITC-labeled, 130- 117-691, clone REA183 | B56 Miltenyi Biotec, USA), CD146 (130- 102-230, clone ME-9F1, Miltenyi Biotec, USA), F4/80 (130-102- 422, REA126 | BM8, Miltenyi Biotec, USA ) at room temperature for 1 hour. .. The dynamics of CD3+ lymphocytes and NK1.1 cells in regenerating livers were analyzed similarly using 105 cells of the liver stromal fraction and primary antibodies to CD45 (PE-labeled, 130-102-596, clone 30F11, Miltenyi Biotec, USA), CD3 (APClabeled, MCA500APC, clone KT3, Bio-Rad USA), NK1.1 (VioBluelabeled, Biolegend, USA); the incubations proceeded for 1 hour.
Article Title: Splenectomy reduces shear stress and inflammation in liver endothelial cells during regeneration after partial hepatectomy in mice
Article Snippet: .. A 10 5 aliquote of LSECs isolated using magnetic sorting for the CD146 marker were incubated in 100 μl Rinsing Solution (Miltenyi Biotec, USA) with 5 μl primary antibodies to CD31 (PECAM-1) (PE-labeled, 130–111-540, clone REA784 | 390, Miltenyi Biotec, USA), integrin alpha-5 (CD49e) (PE-labeled, 130–122-072, clone REA1183 | 5H10-27, Miltenyi Biotec, USA), VCAM-1 (CD106) (PE-labeled, 130–116-323, clone REA971 | 429, Miltenyi Biotec, USA), VE-cadherin (CD144) (PE-labeled, 130–128-207, clone REA225 | BV13, Miltenyi Biotec, USA), Ki67 (FITC-labeled, 130–117-691, clone REA183 | B56 Miltenyi Biotec, USA), CD146 (130–102-230, clone ME-9F1, Miltenyi Biotec, USA), F4/80 (130–102-422, REA126 | BM8, Miltenyi Biotec, USA ) at room temperature for 1 h. The dynamics of CD3 + lymphocytes and NK1.1 cells in regenerating livers were analyzed similarly using 10 5 cells of the liver stromal fraction and primary antibodies to CD45 (PE-labeled, 130–102-596, clone 30F11, Miltenyi Biotec, USA), CD3 (APC-labeled, MCA500APC, clone KT3, Bio-Rad USA), NK1.1 (VioBlue-labeled, Biolegend, USA); the incubations proceeded for 1 h. Following the incubations, the cells were washed in PBS, resuspended in 0.5 ml PBS and analyzed in a MACSQuant 10 flow cytometer (Milteniy Biotech, Germany); the data were analyzed in FlowJo (LLC). ..
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